Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Lu-Chu Ke
Date created: 2011-10-02 11:00:00
Date modified: 2015-05-08 01:12:44
R0011+acsAB+acsCD+CMCax+Ccp/pSB1C3
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K571007_sequence (Version 1) |
Description
The biobrick consists of complete acs operon gene with CMCax and Ccp gene and a promoter which will activate the operon when it is induced by IPTG, cellulose synthase will be synthesized, which later use to produce cellulose.Notes
1.The sequence has a few restriction enzyme cutting sites, therefore checking is needed when we do enzyme digestion and ligation so that the sequence is correctly connected.2.It is a very long sequence, below is few measures to take note during cloning.
*The concentration agarose gel during gel electrophoresis should be about 0.8-1%
*The enzyme digestion reaction time is advised to be elongated so that digestion could be more perfect.
*The respective restriction enzyme at the both end of primer should not cut the sequence of another gene
Source
*organism: Gluconacetobacter hansenii ATCC 23769*acs operon: GenBank AB091060.1
*CMCax: GenBank EFG85213.1
*Ccp: GenBank AAA16970.
| Access | Instance | Definition |
| public public public public | BBa_K571004 BBa_K571000 BBa_K571003 BBa_K571002 | BBa_K571004 BBa_K571000 BBa_K571003 BBa_K571002 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K571004 BBa_K571000 BBa_K571003 BBa_K571002 | 1,80 87,4739 4746,9108 9115,11206 | BBa_K571004 BBa_K571000 BBa_K571003 BBa_K571002 |