BBa_K577889
BBa_K577889 Version 1 Component
This part has been discontinued.
Please consider using BBa_K206004 as a replacement.
Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Alberto Purwada
Date created: 2011-09-10 11:00:00
Date modified: 2015-05-08 01:12:46
pBad+RFPc
| Types | DnaRegion |
| Roles | engineered_region Reporter |
| Sequences | BBa_K577889_sequence (Version 1) |
Description
This reporter part is a combination of a pBad promoter (inducible with arabinose) and a RFP gene. The purpose of this part is to provide the user with a simple, but complete, inducible genetic device. This is useful if the user plans to build a larger plasmid construct in a short time or characterize a specific part. But It is important for the user to determine if his or her cell line contain AraC gene, since AraC gene is required to actually induce pBad promoter with arabinose. See BBa_K577882 or BBa_I13458 for more information.Notes
The RBS, RFP, and Terminator were taken from the BBa_J61002 backbone using SpeI and PstI restriction enzymes.Source
This part comes from ligating 2 biobricks.| Access | Instance | Definition |
| public public public public | BBa_I13453 BBa_B0034 BBa_E1010 BBa_B0015 | BBa_I13453 BBa_B0034 mRFP1 BBa_B0015 |