| Types | DnaRegion
|
| Roles | Reporter
engineered_region
|
| Sequences | BBa_K577893_sequence (Version 1)
|
Description
A composite part containing the TetR gene, C0040, turned on by a constitutive promoter, I14033. The TetR represses the pTet promoter in front of the GFP gene. By addition of tetracycline or aTc, this repression is inhibited and the cells should fluoresce green.
Notes
The whole part is in the backbone of I14033. Both P0440, the TetR composite part, and E0240, the GFP composite part, were used and inserted into their respective promoters. The pTet+GFP part was then combined as a suffix insertion to the I14033+TetR backbone.
Source
Spring 2011 iGEM Kit Plates
