| Types | DnaRegion
|
| Roles | engineered_region
Reporter
|
| Sequences | BBa_K577894_sequence (Version 1)
|
Description
A composite part containing the TetR gene, C0040, turned on by a constitutive promoter, I14033. The TetR represses the pTet promoter in front of the YFP gene. By addition of tetracycline or aTc, this repression is inhibited and the cells should fluoresce yellow.
Notes
The whole part is in the backbone of I14033. Both P0440, the TetR composite part, and E0430, the YFP composite part, were used and inserted into their respective promoters. The pTet+YFP part was then combined as a suffix insertion to the I14033+TetR backbone.
Source
Spring 2011 iGEM Kit Plates
