BBa_K577894

BBa_K577894 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K577894
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Kyle Jones
Date created: 2011-09-14 11:00:00
Date modified: 2015-05-08 01:12:46

TetR repressed YFP



Types
DnaRegion

Roles
engineered_region

Reporter

Sequences BBa_K577894_sequence (Version 1)

Description

A composite part containing the TetR gene, C0040, turned on by a constitutive promoter, I14033. The TetR represses the pTet promoter in front of the YFP gene. By addition of tetracycline or aTc, this repression is inhibited and the cells should fluoresce yellow.

Notes

The whole part is in the backbone of I14033. Both P0440, the TetR composite part, and E0430, the YFP composite part, were used and inserted into their respective promoters. The pTet+YFP part was then combined as a suffix insertion to the I14033+TetR backbone.

Source

Spring 2011 iGEM Kit Plates

igem#experience
None
 
igem#partStatus
Released HQ 2013
igem#sampleStatus
In stock
igem#status
Available
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K577894/1