BBa_K584028

BBa_K584028 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K584028
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Bakul Jitendra Vinchhi, Sarah Veugelen, Katrien Vandermeeren, Yana Hoorne
Date created: 2011-09-18 11:00:00
Date modified: 2015-05-08 01:12:47

pLac-Lux hybrid promoter + INP(Ice Nucleating Protein Generator)



Types
DnaRegion

Roles
engineered_region

Generator

Sequences BBa_K584028_sequence (Version 1)

Description

The pLac-Lux hybrid promoter is repressed by the lacI repressor and induced by IPTG. It is repressed by the luxR repressor bound to the corepressor CO6HSL. It contains a lux box and a lacI binding site. The "Design Notes" portion of this page contains a truth table for this logic gate.

The ice nucleation protein (INP) is a glycosyl phosphatidylinositol anchored outer membrane protein found in certain Gram-negative bacteria. inaZ is the gene responsible for producing the ice nucleation protein.

We would like to thank Prof. Dr. Gregory Gloor , for providing us with a plasmid (pUC1813ICE) which contains the ice nucleating protein gene inaZ.

To know more about the inaZ, it is suggested to read this article describing the usage of pUC1813ICE with the inaZ.

igem#experience
None
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K584028/1