BBa_K592028

BBa_K592028 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K592028
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Lei Sun
Date created: 2011-09-20 11:00:00
Date modified: 2015-05-08 01:12:49

T5-lac promoter-B0032-YFP



Types
DnaRegion

Roles
engineered_region

Measurement

Sequences BBa_K592028_sequence (Version 1)

Description

The promoter T5-lac (BBa_K592008) tested for promoter strength coupled with YFP (BBa_E0034). This construct should be cloned into pSB3K3 plasmid to measure the YFP output and thereby determine the promoter strength. Theoretically, the promoter should be well-repressed in LacIq strain but inducible by high concentrations of IPTG.

Notes

B0032 was picked as per standard. The part was assembled into pSB3K3 for testing and cloned into pSB1C3 for DNA submission.

Source

T5-lac promoter is a fusion protein with parts from the T5 phage promoter.

Access Instance Definition
public
public
public
BBa_K592008
BBa_B0032
BBa_E0034
PT5lac
BBa_B0032
EYFP
igem#experience
Works
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K592028/1