Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Erik Gullberg, Antonio Ascue Avalos, Erik Lundin
Date created: 2011-09-20 11:00:00
Date modified: 2015-05-08 01:12:49
Low to medium copy BioBrick standard vector
| Types | DnaRegion |
| Roles | Plasmid_Backbone plasmid_vector |
| Sequences | BBa_K592200_sequence (Version 1) |
Description
BBa_K592200 is a BioBrick standard vector with low to medium copy p15A replication origin (BBa_I50032) and spectinomycin antibiotic resistance marker, usable for Lambda Red recombineering in E coli. The E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22.Notes
To make the plasmid usable for Lambda Red recombineering, all sequences with homologies to the E coli chromosome had to be removed. To accomplish this, the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22.Source
Salmonella phage P22 late terminator.| Sequence Annotation | Location | Component / Role(s) |
| Translational stop BBa_B0042 Terminator BBa_B0054 VR site BBa_G00102 Terminator BBa_B0062 p15A low to medium copy replication origin BBa_I50032 Lambda red F primer Spectinomycin resistance marker SpcR promoter P22 late terminator VF2 site BBa_G00100 T3 terminator BBa_B0055 Translational stop BBa_B0042 Lambda Red R primer | 35,46 47,115 116,135 136,176 184,1748 1726,1745 1841,2840 2907,2933 2952,2992 2993,3012 3013,3090 3091,3102 20,41 | feature/stop stop_codon feature/misc sequence_feature feature/primer_binding primer_binding_site sequence_feature feature/misc sequence_feature feature/misc primer_binding_site feature/primer_binding feature/cds CDS promoter feature/promoter feature/misc sequence_feature primer_binding_site feature/primer_binding feature/misc sequence_feature feature/stop stop_codon feature/primer_binding primer_binding_site |