Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ceren Seref
Date created: 2011-09-20 11:00:00
Date modified: 2015-05-08 01:12:49
ROSE regulated kill switch
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K593009_sequence (Version 1) |
Description
This composite part consists of promoter, RNA thermometer, GFP and lysis casette. The upstream part of this composite is T7 promoter (BBa_I712074) which is strong promoter from T7 bacteriophage. For strong transcription T7 promoter expression system is chosen. In downstream of promoter there is RNA thermometer(ROSE) coding region which determines the transcription of our lysis composite part. RNA thermometer (BBa_K115001) is temperature sensitive that at 42C translation initiates. That means, up to 42 C RNA thermometer is in dimer form and at 42C it becomes linear. In downstream of RNA thermometer, green fluorescence protein(BBa_E0040) is ligated. This ligation is done to measure expression level and to comment on RNA thermometer efficiency. The last ligate to this composite is bacteriophage 21 lysis casette (BBa_K124003). This lysis casette induces lysis in E.coli. This gene cassette consists of the S protein (Holin), R protein (Endolysin), and Rz protein. When translation initiates at 42 C then the following coding gene begins to produce the proteins which lyse the cell.Notes
No problems occured during assembly process. All parts were compatible with assembly standard 10, so ther weren't any complications about ligating these parts. Also there weren't any problems observed about translation.Source
All parts that produce that composite part come from 2011 kit plate distribution.| Access | Instance | Definition |
| public public public public | BBa_I712074 BBa_K115001 BBa_E0040 BBa_K124003 | BBa_I712074 BBa_K115001 GFP BBa_K124003 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_I712074 BBa_K115001 BBa_E0040 BBa_K124003 | 1,46 55,150 157,876 885,2157 | BBa_I712074 BBa_K115001 GFP BBa_K124003 |