Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Yu Xiaoyan
Date created: 2011-09-30 11:00:00
Date modified: 2015-05-08 01:12:49
A device that can accepts the 3--O-C6-HSL and then produces 3-O-C12-HSL and ECFP reporter.
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K594011_sequence (Version 1) |
Description
This composite is a device of function. it is composed of plac(R0011), RBS(B0034), luxR(C0062), double terminator(B0015), plux(R0062), RBS(B0030), lasI(C0078), RBS(B0034), leuB(K594002), RBS(B0034), ECFP(E0030), double terminator(B0015).When IPTG is added, the plac is activated, producing luxR, and then the plux will be activated when 3-O-C6-HSL is added. The 3-O-C6-HSL units with luxR and form a complex that can bind to the lux cassette in plux so that the transcription of lasI, leuB and ECFP happen, producing 3-O-C12-HSL,leuB and the ECFP reporter.
Notes
When ligate the RBS with others, 3A assembly is not as efficient as standard assembly.Source
Besides the leuB from E.coli BL21 cloned with PCR by ourselves, all the other parts are from the iGEM, including F2622, K081009, B0034, E0030, B0015.| Access | Instance | Definition |
| public public public public public public public | BBa_F2622 BBa_K081009 BBa_B0034 BBa_K594002 BBa_B0034 BBa_E0030 BBa_B0015 | BBa_F2622 BBa_K081009 BBa_B0034 leuB BBa_B0034 eyfp BBa_B0015 |