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BBa_K604000

BBa_K604000 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K604000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Ruojun Wang

Date created: 2011-10-03 11:00:00

Date modified: 2015-05-08 01:12:50

autoinducer synthetase rhlI and GFP under PyeaR

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Details

Types	DnaRegion
Roles	engineered_region Composite
Sequences	BBa_K604000_sequence (Version 1)

Description

The pathway detects nitrates in the medium; if KNO3 is present, the circuit is activated, RhlI produces the AHL (C4-homoserine lactone) and TetR is synthesized to block another pathway. GFP is emitted to show the start of transcription.

Notes

Use low copy number plasmid.

Source

BioBricks

Components

Access	Instance	Definition
public public public public public public public public	BBa_K216005 BBa_B0034 BBa_C0070 BBa_B0034 BBa_C0041 BBa_B0034 BBa_E0040 BBa_B0014	BBa_K216005 BBa_B0034 rhII BBa_B0034 tetR BBa_B0034 GFP BBa_B0014

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K216005 BBa_B0034 BBa_C0070 BBa_B0034 BBa_C0041 BBa_B0034 BBa_E0040 BBa_B0014	1,100 109,120 127,793 802,813 820,1445 1454,1465 1472,2191 2200,2294	BBa_K216005 BBa_B0034 rhII BBa_B0034 tetR BBa_B0034 GFP BBa_B0014

Other Properties

igem#experience	None
igem#sampleStatus	Not in stock
igem#status	Unavailable
synbiohub#ownedBy	user/james
synbiohub#ownedBy	user/myers
synbiohub#topLevel	BBa_K604000/1