Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Olesja Popow
Date created: 2011-09-17 11:00:00
Date modified: 2015-05-08 01:12:52
Prm_cI-DAS_antiPlasB with 0.6RBS
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K607017_sequence (Version 1) |
Description
cI with moderately fast tag under transcriptional control of Prm promoter and reverse antisense lasB promoter, the RBS has a strength of 0.6 (obtained by Jason Kelly and Robbie Bryant during the summer of 2004 using a fluorescent reporter). This construct represents an auto-inducing loop in which the Prm promoter drives expression of its own inducer cI. The running loop can be turned off by activating the reverse antisense lasB promoter (through LasR protein in the presence of Pseudomonas autoinducer (PAI))Notes
We decided to keep PAI concentrations in the media constant (by adding N-(3-oxododecanoyl)homoserine lactone) and control the expression of LasR protein in order to achieve tight control of the reverse antisense lasB promoter.Source
2011 distribution| Access | Instance | Definition |
| public public public public public public public | BBa_B0025 BBa_I12006 BBa_B0030 BBa_K607015 BBa_M0052 BBa_K607008 BBa_B0015 | BBa_B0025 Prm + BBa_B0030 BBa_K607015 BBa_M0052 BBa_K607008 BBa_B0015 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_B0025 BBa_I12006 BBa_B0030 BBa_K607015 BBa_M0052 BBa_K607008 BBa_B0015 | 1,129 138,219 228,242 249,959 968,1000 1009,1109 1118,1246 | BBa_B0025 Prm + BBa_B0030 BBa_K607015 BBa_M0052 BBa_K607008 BBa_B0015 |