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BBa_K611016

BBa_K611016 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K611016

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Nicholas Csicsery

Date created: 2011-09-17 11:00:00

Date modified: 2015-05-08 01:12:53

LacI Repressor and Promoter Wild Type Control

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Details

Types	DnaRegion
Roles	engineered_region Composite
Sequences	BBa_K611016_sequence (Version 1)

Description

This part is can be used as a control for BBa_K611010 and BBa_K611013, which test either C0012 and R0010 mutants respectively.

Notes

There is no terminator at the end, so the part needs to be used in a plasmid that has a terminator after the BioBrick suffix (such as pSB1A3 or pSB1C3). This part contains the PBad promoter without AraC, thus, a strain that already includes AraC is recommended in order to induce PBad.

Source

Built from existing parts in the registry though BioBrick assembly.

Components

Access	Instance	Definition
public public public public public public public	BBa_R0010 BBa_B0034 BBa_E0040 BBa_B0015 BBa_I13453 BBa_B0034 BBa_C0012	LacI BBa_B0034 GFP BBa_B0015 BBa_I13453 BBa_B0034 lacI

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0010 BBa_B0034 BBa_E0040 BBa_B0015 BBa_I13453 BBa_B0034 BBa_C0012	1,200 209,220 227,946 955,1083 1092,1221 1230,1241 1248,2375	LacI BBa_B0034 GFP BBa_B0015 BBa_I13453 BBa_B0034 lacI

Other Properties

igem#partStatus	Released HQ 2013
igem#sampleStatus	In stock
igem#status	Available
synbiohub#ownedBy	user/james
synbiohub#ownedBy	user/myers
synbiohub#topLevel	BBa_K611016/1