Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Nicholas Csicsery
Date created: 2011-09-26 11:00:00
Date modified: 2015-05-08 01:12:53
LacI Promoter Variant #5 in Characterization Construct
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K611035_sequence (Version 1) |
Description
This part contains a variant of the wild type LacI repressible promoter, and is followed by the LacI characterization construct, K611013. This allows for testing of this particular mutant promoter at various concentrations of repressor. The mutated LacI promoter in this plasmid expresses GFP and is followed by an arabinose inducible promoter, pBad, which expresses the LacI repressor. Thus, a user can increase the concentration of repressor by inducing pBad with arabinose. Similarly, the repressor may be unrepressed with the addition of IPTG. This plasmid is very useful for the further characterization of K611025.Notes
This part does not have a termination sequence at the end. It is recommended that for optimal results, this part be used in a backbone that includes a terminator (such as pSB1C3).Source
Mutagenic PCR of the LacI repressible promoter, R0010, and standard BioBrick assembly of parts from the registry.| Access | Instance | Definition |
| public public | BBa_K611025 BBa_K611013 | BBa_K611025 BBa_K611013 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K611025 BBa_K611013 | 1,200 209,2400 | BBa_K611025 BBa_K611013 |