BBa_K611083

BBa_K611083 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K611083
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Timothy Fenton
Date created: 2012-08-20 11:00:00
Date modified: 2015-05-08 01:12:53

BBa_C0051 Test Construct +1(TSS) delete



Types
DnaRegion

Roles
Measurement

engineered_region

Sequences BBa_K611083_sequence (Version 1)

Description

This part is identical to the BBa_C0051 Test Construct (BBa_K611061) except the transcriptional start site has been deleted. This is a thymine base immediately following the barcode and immediately preceding the SpeI site.

Notes

none

Source

Parts Registry

Sequence Annotation Location Component / Role(s)
SpeI
BBa_J61002
BBa_B0034 RBS
mRFP
BBa_B0015
rrnB terminator
NotI
PstI
Verification reverse (VR) primer binding site
ColE1 origin
pBR322 origin
beta-lactamase ampicillin resistance marker
beta-lactamase (ampR, bla) promoter
Verification forward (VF2) primer binding site
BioBrick prefix
Ambiguity; should be a T
BBa_K611063
Truncated cI Lamda Missing ATG Start Codon
LVA
Barcode
1,6
1,2947
8,19
26,706
740,868
751,794
877,884
884,889
947,966
1048,1730
1051,1670
1825,2685
2727,2755
2808,2827
2925,2946
2940,2940
2956,3727
2956,3663
3664,3696
3703,3727
sequence_feature feature/dna
engineered_region feature/BioBrick
engineered_region feature/BioBrick
CDS feature/cds
engineered_region feature/BioBrick
stem_loop feature/stem_loop
feature/dna sequence_feature
feature/dna sequence_feature
feature/primer_binding primer_binding_site
feature/dna sequence_feature
sequence_feature feature/dna
CDS feature/cds
promoter feature/promoter
feature/primer_binding primer_binding_site
feature/dna sequence_feature
feature/mutation sequence_alteration
feature/BioBrick engineered_region
feature/dna sequence_feature
tag feature/tag
feature/barcode engineered_tag
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K611083/1