Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Naomi Genuth
Date created: 2011-09-25 11:00:00
Date modified: 2015-05-08 01:12:54
E. coli strain for TolC one-hybrid selection system
| Types | DnaRegion |
| Roles | chromosome Cell |
| Sequences | BBa_K615003_sequence (Version 1) |
Description
This strain is designed to test zinc finger binding by tying the binding event to cell survival, specifically the expression of TolC, a membrane pump that removes toxins from the cell.Notes
rpoZ knocked out using lambda redKanamycin cassette-Zif268 binding site-weak promoter placed upstream of endogenous TolC
Source
Based off EcNR2 selection strain (see Wang et al, 2009)| Sequence Annotation | Location | Component / Role(s) |
| kanamycin cassette target and promoter region Zif268 binding site | 1,950 951,1046 978,986 | feature/misc sequence_feature promoter feature/promoter non_covalent_binding_site feature/binding |