BBa_K615004

BBa_K615004 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K615004

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Naomi Genuth

Date created: 2011-09-25 11:00:00

Date modified: 2015-05-08 01:12:54

Zif268 binding site-His3-URA3 construct for one-hybrid selection

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K615004_sequence (Version 1)

Description

This is one of the two components necessary for a metabolic His3-URA3 selection system (the other being a zinc finger fused to the omega subunit of RNA polymerase).

Notes

Zif268 binding site in the promoter region to initiate expression of His3 and URA3 only when a zinc finger is bound properly.

Source

The sequence was synthesized.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
target and weak promoter	1,96	promoter, feature/promoter
Zif268 binding site	28,36	non_covalent_binding_site, feature/binding
His3	130,792	feature/protein, CDS
URA3	840,1640	feature/protein, CDS

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K615004/1

Member of these Collections

• iGEM Parts Registry