BBa_K625001

BBa_K625001 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K625001

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Michael Eichenberger

Date created: 2011-09-20 11:00:00

Date modified: 2015-05-08 01:12:56

pTet - LacIM1 - Ter

Details

• Types: DnaRegion
• Roles: Composite; engineered_region
• Sequences: BBa_K625001_sequence (Version 1)

Description

Contains RBS-LacIM1 (BBa_K625000) behind the Tet-Promoter (BBa_R0040)

Notes

As we want a multiplasmid system with LacI behind a Tet and a lambda promoter, we used the codon modified LacIM1 from (Basu et al, 2005) to prevent recombination between the two.

Source

Synthesized by GeneArt. Cloned into a BioBrick backbone.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0040	1,54	feature/BioBrick, engineered_region
TetR 1	1,19	non_covalent_binding_site, feature/binding
-35	20,25	promoter, feature/promoter
TetR 2	26,44	non_covalent_binding_site, feature/binding
-10	43,48	promoter, feature/promoter
RBS	55,89	feature/rbs, ribosome_entry_site
LacI M1	90,1175	CDS, feature/cds
BBa_K625000	55,1175	engineered_region, feature/BioBrick
BBa_B0010	1184,1263	engineered_region, feature/BioBrick
stem_loop	1195,1238	stem_loop, feature/stem_loop
BBa_B0012	1272,1312	engineered_region, feature/BioBrick
T7 TE	1279,1298	feature/stem_loop, stem_loop
polya	1299,1312	polyA_site, feature/polya
stop	1305,1305	feature/stop, stop_codon
BBa_B0015	1184,1312	feature/BioBrick, engineered_region

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K625001/1

Member of these Collections

• iGEM Parts Registry