Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Michael Eichenberger
Date created: 2011-09-20 11:00:00
Date modified: 2015-05-08 01:12:56
pTet - LacIM1 - Ter
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K625001_sequence (Version 1) |
Description
Contains RBS-LacIM1 (BBa_K625000) behind the Tet-Promoter (BBa_R0040)Notes
As we want a multiplasmid system with LacI behind a Tet and a lambda promoter, we used the codon modified LacIM1 from (Basu et al, 2005) to prevent recombination between the two.Source
Synthesized by GeneArt. Cloned into a BioBrick backbone.| Sequence Annotation | Location | Component / Role(s) |
| BBa_R0040 TetR 1 -35 TetR 2 -10 RBS LacI M1 BBa_K625000 BBa_B0010 stem_loop BBa_B0012 T7 TE polya stop BBa_B0015 | 1,54 1,19 20,25 26,44 43,48 55,89 90,1175 55,1175 1184,1263 1195,1238 1272,1312 1279,1298 1299,1312 1305,1305 1184,1312 | feature/BioBrick engineered_region non_covalent_binding_site feature/binding promoter feature/promoter non_covalent_binding_site feature/binding promoter feature/promoter feature/rbs ribosome_entry_site CDS feature/cds engineered_region feature/BioBrick engineered_region feature/BioBrick stem_loop feature/stem_loop engineered_region feature/BioBrick feature/stem_loop stem_loop polyA_site feature/polya feature/stop stop_codon feature/BioBrick engineered_region |