Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Spencer Scott
Date created: 2011-08-31 11:00:00
Date modified: 2015-05-08 01:12:58
P_rffGH.rbs.ToxR-Nhe1.{gsgsgs}{LambdaRep}
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K641007_sequence (Version 1) |
Description
This composite part constitutively expresses a ToxR-LambdaRep construct. This ToxR construct will dimerize at a high affinity, because of Lambda Rep's dimerizing properties, making the cytoplasmic ToxR dimer an active transcription factor for the ctx Promoter. Thus, If this composite part is combined with a Pctx-GFP reporter there will be a high expression of GFP.Notes
The biggest design considerations were how to limit the amount of ToxR expressed while still allowing enough ToxR to be produced to see a high expression of GFP. The P_rffGH promoter, the rbs, and the plasmid we put it on (ColE1 origin) all contributed to the optimal expression level of the ToxR construct.Source
This composite part comes from several sources all listed in its respective basic parts.| Access | Instance | Definition |
| public public public | BBa_K641004 BBa_K641005 BBa_K641006 | BBa_K641004 BBa_K641005 BBa_K641006 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K641004 BBa_K641005 BBa_K641006 | 1,25 32,685 694,1434 | BBa_K641004 BBa_K641005 BBa_K641006 |