Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Wilson Lam, Matthew Orton
Date created: 2011-09-21 11:00:00
Date modified: 2015-05-08 01:12:58
TetR repressor tagged with yBFP and a VP16 activation domain
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K642004_sequence (Version 1) |
Description
Tetracycline inducible repressor (no stop codon included) tagged upstream with yeast codon optimized blue fluorescent protein (no stop codon included) and a VP16 activation domain.Notes
Both TetR and yeast codon optimized BFP were designed without a stop codon to enable the construction of a fusion protein with the VP16 activation domain.Source
The TetR repressor was PCR amplified from a plasmid source of a biobrick (BBa_K319046) that was submitted last year by the uOttawa team. Yeast codon optimized BFP was synthesized by the uOttawa team using Mr. gene. The VP16 activation domain was PCR amplified from a plasmid.| Sequence Annotation | Location | Component / Role(s) |
| TetR ORF XbaI/SpeI mixed site BFP ORF XbaI/SpeI mixed site VP16 Activation domain | 1,621 622,627 628,1326 1327,1332 1333,1718 | CDS feature/cds sequence_feature feature/misc CDS feature/cds feature/misc sequence_feature CDS feature/cds |