BBa_K642005

BBa_K642005 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K642005

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Wilson Lam, Matthew Orton

Date created: 2011-09-22 11:00:00

Date modified: 2015-05-08 01:12:58

LacI repressor tagged with yBFP and a VP16 activation domain

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K642005_sequence (Version 1)

Description

LacI repressor tagged upstream with yeast codon optimized blue fluorescent protein and an rtTA activation domain.

Notes

Both TetR and yeast codon optimized BFP were designed without a stop codon to enable the construction of a fusion protein with BFP and the rtTA activation domain.

Source

The LacI repressor was PCR amplified from a plasmid source of a biobrick (BBa_K319046) that was submitted last year by the uOttawa team. Yeast codon optimized BFP was synthesized by the uOttawa team using Mr. Gene DNA synthesis. The rtTA activation domain was PCR amplified from a plasmid obtained by our host lab.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
LacI ORF	1,1086	feature/cds, CDS
XbaI/SpeI mixed site	1087,1092	feature/misc, sequence_feature
BFP ORF	1093,1791	feature/cds, CDS
XbaI/SpeI mixed site	1792,1797	feature/misc, sequence_feature
VP16 Activation domain	1798,2183	feature/cds, CDS

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K642005/1

Member of these Collections

• iGEM Parts Registry