Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: James Coletta, Anisha Katyal, Kristen Salavo
Date created: 2011-07-24 11:00:00
Date modified: 2015-05-08 01:12:59
Or with RBS B0034 and Mcherry
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K648030_sequence (Version 1) |
Description
The Operating region of the lambda phage switch system with Mcherry on a strong RBS attached after the pr promoter of Or. We used this part in our project to test the pr promoter in Or. Since Mcherry has RFP, one can measure the translation rate of Mcherry. Theoretically you could attach C1 to the prm promoter of Or in order to test its repression and no fluoresence should be observed.Source
We recieved Mcherry from our graduate student advisor Mike Speer. B0034 was taken from the 2011 registry. Or sequencing was found in Gaylen Lynch's notebook (Penn State iGEM 2007 member) and constructed with primers.| Access | Instance | Definition |
| public public public | BBa_K648029 BBa_B0034 BBa_J06505 | BBa_K648029 BBa_B0034 mCherry |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K648029 BBa_B0034 BBa_J06505 | 1,82 91,102 109,831 | BBa_K648029 BBa_B0034 mCherry |