Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Yidan Hu
Date created: 2011-09-30 11:00:00
Date modified: 2015-05-08 01:13:02
a bacteria population-control device with RBS0.3 driven by lacl+pL
| Types | DnaRegion |
| Roles | engineered_region Device |
| Sequences | BBa_K658004_sequence (Version 1) |
Description
The device is a twin part of Biobrick part BBa_K658001. This device is made up of three subparts: a LuxI produce, a LuxR producer and a killer protein producer. It is designed to build a programmed bacterial death circuit, which is based on the quorum sensing system of Vibrio fischeri. iGEM-Team XMU-China has designed a series of bacteria population-control devices using RBSs of different strength in the killer protein producer. This device has the ability to maintain cell density of the bacteria population at a relatively low value compared with bacteria without this circuit and extends the stable stage. RBS0.3 was used to regulate the expression of killer protein ccdB.Notes
This device is based on BBa_K658001. The RBS0.3 (BBa_B0032) in the killer protein producer regulate the expression of killer protein LacZalpha-ccdB. The higher strength a RBS has, the lower cell density may be got in the steady state.Source
BBa_K658001| Access | Instance | Definition |
| public public public public public public | BBa_R0011 BBa_F1610 BBa_K658000 BBa_B0032 BBa_K176003 BBa_B0015 | lacI+pL BBa_F1610 BBa_K658000 BBa_B0032 lacZa-ccdB BBa_B0015 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_R0011 BBa_F1610 BBa_K658000 BBa_B0032 BBa_K176003 BBa_B0015 | 1,54 64,861 870,1931 1940,1952 1959,2438 2447,2575 | lacI+pL BBa_F1610 BBa_K658000 BBa_B0032 lacZa-ccdB BBa_B0015 |