BBa_K676777

BBa_K676777 Version 1

Component

This part has been discontinued.

Please consider using BBa_K185030 as a replacement.

Source: http://parts.igem.org/Part:BBa_K676777

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Kheng Tee Ng

Date created: 2011-09-16 11:00:00

Date modified: 2015-05-08 01:13:04

GyrA cassette

Details

• Types: DnaRegion
• Roles: Composite; engineered_region
• Sequences: BBa_K676777_sequence (Version 1)

Description

This expression cassette is regulated by an IPTG inducible promoter and will be expressing Gyr A subunits . Gyr A subunit will bind to the DNA ( gyrase binding site) and is responsible for catalysing DNA breakage and resealing in order to change the level of supercoiling in DNA. However in order to introduce negative supercoiling into the DNA , a functional tetrameric gyrase structure with two GyrA and two GyrB should form with the presence of divalent magnesium ions and ATP.

Notes

The wild type Gyr A ORF has a Pst 1 restriction site at the 1212th bp position so site directed mutagenesis is carried out to remove the restriction site.

Source

Gyr A gene is obtained from E.coli genome.

Components

Access	Instance	Definition
public	BBa_B0015	BBa_B0015
public	BBa_R0010	LacI
public	BBa_B0034	BBa_B0034

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_B0015	1,129	BBa_B0015
BBa_R0010	138,337	LacI
BBa_B0034	346,357	BBa_B0034

Other Properties

• igem#experience: None
• igem#sampleStatus: Discontinued
• igem#status: Deleted
• isReplacedBy: BBa_K185030/1
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K676777/1

Member of these Collections

• iGEM Parts Registry