BBa_K726012

BBa_K726012 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K726012

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Kendall Kearns

Date created: 2012-10-01 11:00:00

Date modified: 2015-05-08 01:13:05

T7Prom+HisTag+YefM

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K726012_sequence (Version 1)

Description

This part contains YefM the antitoxin of the yefM-yoeB toxin-antitoxin system. It is behind a T7 Promoter with a , lac operator, ribosome binding site, and 6x his tag. The construct can be moved to any plasmid for expression.

Notes

n/a

Source

YefM was cloned from E. coli gDNA and the rest of the part is from the Novagen pcdfDuet plasmid.

Components

Access	Instance	Definition
public	BBa_K726003	BBa_K726003
public	BBa_K726006	BBa_K726006

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K726003	1,129	BBa_K726003
BBa_K726006	130,381	BBa_K726006

Other Properties

• igem#experience: None
• igem#sampleStatus: In stock
• igem#status: Available
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K726012/1

Member of these Collections

• iGEM Parts Registry