Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Andrea Tassinari, Francesco Guzzonato
Date created: 2012-08-21 11:00:00
Date modified: 2015-05-08 01:13:06
IPTG Inducible sfGFP tagged Cysteine desulfhydrase (CysDes)
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K731480_sequence (Version 1) |
Description
This composite part is the same as BBa_K731400 but with the add of a sfGFP (superfolder GFP) downstream. This reporter gene is very useful to check and measure the expression of an upstream gene, that in this case codes for a cysteine desulfhydrase; its role is converting cysteine into pyruvate, ammonia and hydrogen sulfide.Notes
The sfGFP is the BioBrick BBa_K515005 designed by iGEM11_Imperial_College_London.Source
LacI+LacIq promoter comes from pET-21b plasmid. CysDes gene comes from the genome of Treponema denticola.| Sequence Annotation | Location | Component / Role(s) |
| LacI stop start LacIq promoter Ptac promoter LacO operator rbs CysDes superfolder GFP coding region start stop | 1,1083 1,3 1081,1083 1093,1152 1161,1190 1195,1219 1249,1255 1263,2459 2478,3194 2478,2480 3192,3194 | feature/cds CDS feature/stop stop_codon feature/start start_codon promoter feature/promoter feature/promoter promoter feature/operator operator ribosome_entry_site feature/rbs feature/cds CDS CDS feature/protein start_codon feature/start feature/stop stop_codon |