BBa_K731520

BBa_K731520 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K731520
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Francesco Guzzonato
Date created: 2012-08-21 11:00:00
Date modified: 2015-05-08 01:13:06

IPTG inducible GFPmut3b



    • Details

    Types
    DnaRegion

    Roles
    engineered_region

    Composite

    Sequences BBa_K731520_sequence (Version 1)

    Description

    This is a construct carrying the sfGFP gene with low-efficiency RBS, under lac operator control.

    lac repressor (encoded from lacI gene) is the regulator protein: in absence of lactose the protein binds to lac operator, preventing RNA-polymerase binding and transcription.Low, constitutive levels of transcription can be seen anyhow.

    Source

    This construct has ben obtained from ligation between BBa_K731500 and BBa_E0240.

    Access Instance Definition
    public
    public
    		BBa_K731500
    BBa_E0240
    		BBa_K731500
    GFP report

    Sequence Annotation Location Component / Role(s)
    BBa_K731500
    BBa_E0240
    		1,1239
    1248,2123
    		BBa_K731500
    GFP report

    igem#experience
    Works
     
    igem#partStatus
    Released HQ 2013
    igem#sampleStatus
    In stock
    igem#status
    Available
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#topLevel
    BBa_K731520/1
     

    Attachments

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