Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Li Kang
Date created: 2012-09-17 11:00:00
Date modified: 2015-05-08 01:13:07
Inducible GVPA Generator
| Types | DnaRegion |
| Roles | engineered_region Generator |
| Sequences | BBa_K737004_sequence (Version 1) |
Description
This part contains a constitutive promoter (J23106), a coding region of LacI protein with an LVA degradation tail, a terminator (B0015), a promoter repressed by LacI protein (R0011) a coding region of GvpA and GFP and a terminator (B0015) behind it.This part can be used to induce the strength of promoter (R0011) by IPTG and indicate the content of GvpA by fluorescence of GFP. In short, it can regulate the expression of GvpA.
We can use different concentration of IPTG to regulate the strength of promoter (R0011) ahead GvpA Protein and GFP. And We can know the approximate concentration of GvpA by the fluorescence unit of GFP.
To achieve this goal, we measured a curve to connect the concentration of IPTG and the fluorescence unit of GFP. Here is the curve:
Notes
We can use different concentration of IPTG to regulate the strength of promoter (R0011) ahead GvpA Protein and GFP. And We can know the approximate concentration of GvpA by the fluorescence unit of GFP.To achieve this goal, we measured a curve to connect the concentration of IPTG and the fluorescence unit of GFP.
Source
No| Access | Instance | Definition |
| public public public public public public | BBa_J23106 BBa_P0412 BBa_R0011 BBa_B0034 BBa_K737016 BBa_E0840 | BBa_J23106 BBa_P0412 lacI+pL BBa_B0034 BBa_K737016 GFP genera |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23106 BBa_P0412 BBa_R0011 BBa_B0034 BBa_K737016 BBa_E0840 | 1,35 44,1351 1360,1413 1423,1434 1441,1659 1668,2545 | BBa_J23106 BBa_P0412 lacI+pL BBa_B0034 BBa_K737016 GFP genera |