Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Li Kang
Date created: 2012-09-17 11:00:00
Date modified: 2015-05-08 01:13:07
Inducible GVPC Generator
| Types | DnaRegion |
| Roles | Generator engineered_region |
| Sequences | BBa_K737005_sequence (Version 1) |
Description
This part contains a constitutive promoter (J23106), a coding region of TetR protein, a terminator (B0015), a promoter repressed by TetR protein (R0040) a coding region of GvpC and GFP and a terminator (B0015) behind it.This part can be used to induce the strength of promoter (R0040) by Tet and indicate the content of GvpC by fluorescence of GFP. In short, it can regulate the expression of GvpC.
We can use different concentration of Tet to regulate the strength of promoter (R0040) ahead GvpC Protein and GFP. And We can know the approximate concentration of GvpC by the fluorescence unit of GFP.
To achieve this goal, we measured a curve to connect the concentration of IPTG and the fluorescence unit of GFP. Here is the curve:
Notes
We can use different concentration of Tet to regulate the strength of promoter (R0040) ahead GvpC Protein and GFP. And We can know the approximate concentration of GvpC by the fluorescence unit of GFP.To achieve this goal, we measured a curve to connect the concentration of IPTG and the fluorescence unit of GFP.
Source
No| Access | Instance | Definition |
| public public public public public public | BBa_J23106 BBa_P0440 BBa_R0040 BBa_B0034 BBa_K737017 BBa_E0840 | BBa_J23106 BBa_P0440 p(tetR) BBa_B0034 BBa_K737017 GFP genera |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23106 BBa_P0440 BBa_R0040 BBa_B0034 BBa_K737017 BBa_E0840 | 1,35 44,883 892,945 954,965 972,1475 1484,2361 | BBa_J23106 BBa_P0440 p(tetR) BBa_B0034 BBa_K737017 GFP genera |