Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Wenjie Wu and Peiran Zhang
Date created: 2012-09-18 11:00:00
Date modified: 2015-05-08 01:13:07
galK::GFP generator ligated to sRNA device controlled by aTc
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K737039_sequence (Version 1) |
Description
Small RNA Spot42 controls the GFP mRNA translation by blocking the RBS in galK.The galK and E0040 is fused together using ClaI site to avoid stop condon in standard scar.J23106 is a moderate promotor,and galK???s RBS is a moderate one.[J23106,P0440]is a TetR generator, which represses tet promotor and can be induced by aTc.
aTc concentration gradient results in different sRNA Spot42 levels in cells, which can be monitored by our galK::GFP generator,where galK is the RBS of GFP and the target for Spot42.
Notes
References:[1]Johannes H. Urban and Jo?? rg Vogel, Translational control and target recognition by Escherichia coli small RNAs in vivo, Nucleic Acids Research, 2007, Vol. 35, No. 3
[2] Boris G??rke and J??rg Vogel, Noncoding RNA control of the making and breaking of sugars, GENES & DEVELOPMENT 22:2914???2925 ,2008
Source
No| Access | Instance | Definition |
| public public public public public public public | BBa_J23106 BBa_P0440 BBa_R0040 BBa_K737058 BBa_J23106 BBa_K737033 BBa_K737066 | BBa_J23106 BBa_P0440 p(tetR) BBa_K737058 BBa_J23106 BBa_K737033 BBa_K737066 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23106 BBa_P0440 BBa_R0040 BBa_K737058 BBa_J23106 BBa_K737033 BBa_K737066 | 1,35 44,883 892,945 954,1062 1071,1105 1114,1240 1249,1965 | BBa_J23106 BBa_P0440 p(tetR) BBa_K737058 BBa_J23106 BBa_K737033 BBa_K737066 |