Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Wenjie Wu and Peiran Zhang
Date created: 2012-09-18 11:00:00
Date modified: 2015-05-08 01:13:07
galK::GFP generator ligated to LacI generator and constitutive sRNA device
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K737041_sequence (Version 1) |
Description
Small RNA Spot42 controls the GFP mRNA translation by blocking the RBS in galK.The galK and E0040 is fused together using ClaI site to avoid stop condon in standard scar.J23106 is a moderate promotor,and galK???s RBS is a moderate one.[J23106,P0412]is a LacI generator, which represses tet promotor and can be induced by IPTG.
aTc concentration gradient results in different sRNA Spot42 levels in cells when presented R0011, which can be monitored by our galK::GFP generator,where galK is the RBS of GFP and the target for Spot42.
This device was mistaken for aTc inducible sRNA device originally due to some ligation mistake,but we actually found this one is perfect to express full level sRNA to verify sRNA function without induction.
Notes
References:[1] Boris G??rke and J??rg Vogel, Noncoding RNA control of the making and breaking of sugars, GENES & DEVELOPMENT 22:2914???2925 ,2008
Source
No| Access | Instance | Definition |
| public public public public public public public | BBa_J23106 BBa_P0412 BBa_R0040 BBa_K737058 BBa_J23106 BBa_K737033 BBa_K737066 | BBa_J23106 BBa_P0412 p(tetR) BBa_K737058 BBa_J23106 BBa_K737033 BBa_K737066 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23106 BBa_P0412 BBa_R0040 BBa_K737058 BBa_J23106 BBa_K737033 BBa_K737066 | 1,35 44,1351 1360,1413 1422,1530 1539,1573 1582,1708 1717,2433 | BBa_J23106 BBa_P0412 p(tetR) BBa_K737058 BBa_J23106 BBa_K737033 BBa_K737066 |