Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Wenjie Wu and Peiran Zhang
Date created: 2012-09-18 11:00:00
Date modified: 2015-05-08 01:13:07
constitutive Spot42 generator ligated to TetR generator
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K737042_sequence (Version 1) |
Description
[J23106,P0440]is a TetR generator, which represses tet promotor and can be induced by aTc.aTc concentration gradient results in different sRNA Spot42 levels in cells when presented R0040, which can be monitored by our galK::GFP generator,where galK is the RBS of GFP and the target for Spot42.
This device was mistaken for IPTG inducible sRNA device originally due to some ligation mistake,but we actually found this one is perfect to express full level sRNA to verify sRNA function without induction.When assembly the corresponding part together(constitutive Spot42 generator ligated to lacI generator),the double input platform will work efficiently.
Notes
References:[1] Boris G??rke and J??rg Vogel, Noncoding RNA control of the making and breaking of sugars, GENES & DEVELOPMENT 22:2914???2925 ,2008
Source
No| Access | Instance | Definition |
| public public public public | BBa_J23106 BBa_P0440 BBa_R0011 BBa_K737058 | BBa_J23106 BBa_P0440 lacI+pL BBa_K737058 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23106 BBa_P0440 BBa_R0011 BBa_K737058 | 1,35 44,883 892,945 955,1063 | BBa_J23106 BBa_P0440 lacI+pL BBa_K737058 |