Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Chunyan Zhang
Date created: 2012-09-18 11:00:00
Date modified: 2015-05-08 01:13:08
Plac-GFP Generator ligated to TetR Generator
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K737053_sequence (Version 1) |
Description
[J23106,P0440]is a TetR generator, which represses tet promotor and can be induced by aTc.IPTG concentration gradient results in different GFP levels in cells when presented lacI generator, which can be monitored plate reader Synergy HT.
This device was mistaken for IPTG inducible sRNA device originally due to some ligation mistake,but we actually found this one is perfect to express full level GFP without induction, so we can exclude the native lacI noise while expressing tetR(some kind of load to cells) in data processing.
When assembly the corresponding part together(Ptet-GFP generator ligated to lacI generator),the double input platform will work efficiently.
Notes
NoSource
No| Access | Instance | Definition |
| public public public public | BBa_J23106 BBa_P0440 BBa_R0011 BBa_E0240 | BBa_J23106 BBa_P0440 lacI+pL GFP report |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23106 BBa_P0440 BBa_R0011 BBa_E0240 | 1,35 44,883 892,945 955,1830 | BBa_J23106 BBa_P0440 lacI+pL GFP report |