Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Wenjie Wu and Peiran Zhang
Date created: 2012-09-18 11:00:00
Date modified: 2015-05-08 01:13:08
YtfJ_s1s2_Gnt
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K737056_sequence (Version 1) |
Description
The leading sequence involved in the YtfJ transcript which can act as the target of the sRNA, spot42. It enables strong competition with the galk::GFP to interact with spot42 which shows great potential for constructing sRNA-mediated circuit.Small RNA Spot42 controls the GFP mRNA translation by blocking the RBS in galK.The galK and E0040 is fused together using ClaI site to avoid stop condon in standard scar.J23106 is a moderate promotor,and galK???s RBS is a moderate one.
Spot42 sRNA is under control of aTc inducible device,the buffer RNA YtfJ is under control of IPTG inducible device.IPTG/aTc concentration gradient results in different YtfJ RNA/sRNA Spot42 levels in cells, which can be monitored by our galK::GFP generator(where galK is the RBS of GFP and the target for Spot42) ,Spot42 can be buffered by YtfJ_Spot42 Scaffold fusion RNA.
Notes
References:[1]Chase L. Beisel and Gisela Storz, The Base-Pairing RNA Spot 42 Participates in a Multioutput Feedforward Loop to Help Enact Catabolite Repression in Escherichia coli. Molecular Cell 41, 286???297, February 4, 2011
Source
No| Access | Instance | Definition |
| public public public public public public public public | BBa_J23106 BBa_P0440 BBa_R0011 BBa_K737049 BBa_J23106 BBa_P0412 BBa_R0040 BBa_K737058 | BBa_J23106 BBa_P0440 lacI+pL BBa_K737049 BBa_J23106 BBa_P0412 p(tetR) BBa_K737058 |