Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Wenjie Wu and Minghao Gong
Date created: 2012-09-18 11:00:00
Date modified: 2015-05-08 01:13:08
GalK_Constitutive anti-messenger RNA generator
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K737062_sequence (Version 1) |
Description
This device mean to construct IPTG/aTc NOR GATE.AntiGalK stands for the RNA transcripted fully complementary to galK,which shows modest repression on galk::GFP mRNA.
Small RNA Spot42 controls the GFP mRNA translation by blocking the RBS in galK.The galK and E0040 is fused together using ClaI site to avoid stop condon in standard scar.J23106 is a moderate promotor,and galK???s RBS is a moderate one.
Spot42 sRNA is under the control of aTc inducible device,another RNA antiGalK is under the control of IPTG inducible device.
Notes
References:[1] Boris G??rke and J??rg Vogel, Noncoding RNA control of the making and breaking of sugars, GENES & DEVELOPMENT 22:2914???2925 ,2008
Source
No| Access | Instance | Definition |
| public public public public public | BBa_J23106 BBa_P0440 BBa_R0011 BBa_K737057 BBa_K737033 | BBa_J23106 BBa_P0440 lacI+pL BBa_K737057 BBa_K737033 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23106 BBa_P0440 BBa_R0011 BBa_K737057 BBa_K737033 | 1,35 44,883 892,945 955,1007 1016,1142 | BBa_J23106 BBa_P0440 lacI+pL BBa_K737057 BBa_K737033 |