psb1C3_int

BBa_K743008 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K743008
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Bernardo Pollak, Isaac Nu??ez
Date created: 2012-09-19 11:00:00
Date modified: 2015-05-08 01:13:09

psb1C3_IntKR recombination plasmid for Synechocystis PCC6803



Types
DnaRegion

Roles
engineered_region

Composite

Sequences BBa_K743008_sequence (Version 1)

Description

This plasmid allows the integration of a double terminator followed by a reversed Kanamycin resistance casette in to Synechocystis chromosome, making possible selection in this cyanobacteria. It has a Chloramphenicol resistance casette for selection in E.coli
The use of BBa_K743000 and BBa_K743001 as recombination sites has no deleterious phenotypic effects on the cells.
The plasmid is intended to be used as a starting backbone for further adition of dna parts by Gibson assembly.
It was used by UC_Chile 2012 team to succesfully transform Synechocystis

Notes

Using a double terminator followed by a reversed antibiotic resistance cds allows the use of the same terminator sequence for both the resistance casette and any desired coding sequence placed upstream

Source

Both BBa_K743001 and BBa_K743002 were PCR amplified from genomic dna. BBa_K743001 and BBa_B0015 were amplified from biobricks.

Access Instance Definition
public
public
public
BBa_K743000
BBa_S05073
BBa_K743001
RS1
BBa_S05073
RS2
igem#experience
Works
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K743008/1