Types | DnaRegion
|
Roles | engineered_region
Composite
|
Sequences | BBa_K743008_sequence (Version 1)
|
Description
This plasmid allows the integration of a double terminator followed by a reversed Kanamycin resistance casette in to Synechocystis chromosome, making possible selection in this cyanobacteria. It has a Chloramphenicol resistance casette for selection in E.coli
The use of
BBa_K743000 and
BBa_K743001 as recombination sites has no deleterious phenotypic effects on the cells.
The plasmid is intended to be used as a starting backbone for further adition of dna parts by Gibson assembly.
It was used by
UC_Chile 2012 team to succesfully transform Synechocystis
Notes
Using a double terminator followed by a reversed antibiotic resistance cds allows the use of the same terminator sequence for both the resistance casette and any desired coding sequence placed upstream