BBa_K759012

BBa_K759012 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K759012
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Kenta Mine
Date created: 2012-09-19 11:00:00
Date modified: 2015-05-08 01:13:13

eCFP + E. coli aggregation (Ag43) device induced by pBAD promoter.



    • Details

    Types
    DnaRegion

    Roles
    Composite

    engineered_region

    Sequences BBa_K759012_sequence (Version 1)

    Description

    In the presence of L-arabinose, pBAD promoter is induced and then wildtype Antigen 43 (Ag43) protein and eCFP (engineered cyan fluorescent protein)is produced.
    The wildtype Ag43 have 6 PstI sites.

    eCFP locates in the upstream of Ag43 for estimating the relationship between expressed protein amount and aggregation efficiency.

    Notes

    6 PstI sites exists in Ag43 coding site so we assembled this composite part without using PstI.

    Source

    Ag43(BBA_K346007) was derived from E. coli K-12 strain by Peking 2010 iGEM.

    igem#partStatus
    Released HQ 2013
    igem#sampleStatus
    In stock
    igem#status
    Available
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K759012/1
     

    Attachments

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