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BBa_K759012

BBa_K759012 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K759012

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Kenta Mine

Date created: 2012-09-19 11:00:00

Date modified: 2015-05-08 01:13:13

eCFP + E. coli aggregation (Ag43) device induced by pBAD promoter.

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Details

Types	DnaRegion
Roles	Composite engineered_region
Sequences	BBa_K759012_sequence (Version 1)

Description

In the presence of L-arabinose, pBAD promoter is induced and then wildtype Antigen 43 (Ag43) protein and eCFP (engineered cyan fluorescent protein)is produced.
The wildtype Ag43 have 6 PstI sites.

eCFP locates in the upstream of Ag43 for estimating the relationship between expressed protein amount and aggregation efficiency.

Notes

6 PstI sites exists in Ag43 coding site so we assembled this composite part without using PstI.

Source

Ag43(BBA_K346007) was derived from E. coli K-12 strain by Peking 2010 iGEM.

Components

Access	Instance	Definition
public public public public public public	BBa_I0500 BBa_B0034 BBa_E0020 BBa_B0034 BBa_K346007 BBa_B0015	pBad/araC BBa_B0034 ecfp BBa_B0034 BBa_K346007 BBa_B0015

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_I0500 BBa_B0034 BBa_E0020 BBa_B0034 BBa_K346007 BBa_B0015	1,1210 1219,1230 1237,1959 1968,1979 1986,5105 5114,5242	pBad/araC BBa_B0034 ecfp BBa_B0034 BBa_K346007 BBa_B0015

Other Properties

igem#partStatus	Released HQ 2013
igem#sampleStatus	In stock
igem#status	Available
synbiohub#ownedBy	user/james
synbiohub#ownedBy	user/myers
synbiohub#topLevel	BBa_K759012/1