BBa_K762001

BBa_K762001 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K762001

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Menuka Samaranayake

Date created: 2012-09-23 11:00:00

Date modified: 2015-05-08 01:13:13

TCC Negative control

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K762001_sequence (Version 1)

Description

Negative control for Translational Coupling Cassette by UW-Madison 2012's team. Based off UW-Madison's BBa_K762100 but with a stop codon in the middle of the sequence. This stop codon will prevent the hairpin from going away, preventing translation and florescence.

Notes

Designed purposely to include a stop codon prematurely in the sequence to prevent translation

Source

Based off of UW-Madison's BBa_K762100

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_J23102	1,35	engineered_region, feature/BioBrick
RBS	44,62	feature/rbs, ribosome_entry_site
Limonene Synthase ORF	63,1727	sequence_feature, feature/misc
Embedded Stop 1	834,836	stop_codon, feature/stop
Embedded Stop 2	867,869	feature/stop, stop_codon
BBa_K762000	1736,2453	feature/BioBrick, engineered_region
His-Tag for fused Target Gene	1736,1753	tag, feature/tag
mRNA Hairpin	1737,1771	stem_loop, feature/stem_loop
Stop Codon for fused Target Gene	1754,1756	stop_codon, feature/stop
RBS for Response Gene	1759,1764	feature/rbs, ribosome_entry_site
Red Florescent Protein ORF	1773,2450	sequence_feature, feature/misc

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K762001/1

Member of these Collections

• iGEM Parts Registry