Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Mustafa Elitok
Date created: 2012-09-25 11:00:00
Date modified: 2015-05-08 01:13:15
EpCAM Detection Device
| Types | DnaRegion |
| Roles | engineered_region Project |
| Sequences | BBa_K772100_sequence (Version 1) |
Description
This gene sequence includes both of EpCAM Binding Docks; BBa_K772001 and BBa_K772002.Thus, this composite part is the main component of CTC (Circulating Tumor Cell) detection system. Docks include an EpCAM (Epithelial Cell Adhesion Molecule) antigen binding domain, OmpA transmembrane protein (dock) and TEV protease C or N terminus. Hopefully, after binding to the EpCAM antigen, TEV protease domains will fix together to form the full structure in order to activate the signalling pathway. (For more information: BBa_K772003)
EpCAM is an natural antigen which is found high significantly in the cases of some breast and colorectal cancer subtypes.
On the upstream region of protein sequences, there are a constitutive pLac promoter and appropriate RBS. To see how this part works: Experience.
Notes
There are two protein sequences between domains and the dock: One between the binding domain (antibody) and dock and one between the domain of TEV protease.Source
CD225 (EpCAM antibody) is commonly used for the test of presence of EpCAM in the laboratories. The sequences of OmpA and TEV protease terminuses are acquired from the Registry.| Access | Instance | Definition |
| public public | BBa_K772001 BBa_K772002 | BBa_K772001 BBa_K772002 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K772001 BBa_K772002 | 1,1159 1168,2305 | BBa_K772001 BBa_K772002 |