Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: NRP-UEA-Norwich
Date created: 2012-09-18 11:00:00
Date modified: 2015-05-08 01:13:15
Bacterial-Mammalian promoter with RFP reporter: BBaK216005 + BHN + CArG promoter sequence E9-ns2 + B
| Types | DnaRegion |
| Roles | Reporter engineered_region |
| Sequences | BBa_K774005_sequence (Version 1) |
Description
Our hybrid promoter hopes to add to the systems already in the registry by creating a hybrid promoter that combines the bacterial promoter PyeaR and the mammalian CArG element , both of which respond to exogenous nitrogenous species. Combining the two would allow a more modular NO sensor that can be used in mammalian and bacterial cells interchangeably. The hybrid promoter has been attached to the reporter: Red Fluorescent Protein (RFP). The hybrid promoter has been characterised by observing expression of flourescent protein, and found to have increased transcription in response to increasing concentrations of potassium nitrate.Notes
This biobrick contains the RBS and terminators meaning we can simply add the promoter of choice to complete the system.Source
BBaK216005 + BHN + CArG promoter sequence E9-ns2 + BBa_K081014| Access | Instance | Definition |
| public public | BBa_K774000 BBa_K081014 | B-M BBa_K081014 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K774000 BBa_K081014 | 1,208 217,990 | B-M BBa_K081014 |