Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: NRP-UEA-Norwich
Date created: 2012-09-18 11:00:00
Date modified: 2015-05-08 01:13:15
Mammalian-Bacterial promoter with RFP reporter: CArG promoter sequence + BBaK216005 + BBa_K08101
| Types | DnaRegion |
| Roles | engineered_region Reporter |
| Sequences | BBa_K774007_sequence (Version 1) |
Description
Our hybrid promoter hopes to add to the systems already in the registry by creating a hybrid promoter that combines the bacterial promoter PyeaR and the mammalian CArG element , in the orientation mammalian to bacterial, both of which respond to exogenous nitrogenous species. Combining the two would allow a more modular NO sensor that can be used in mammalian and bacterial cells interchangeably. The hybrid promoter has been attached to the reporter: Red Fluorescent Protein (RFP). The hybrid promoter has been characterised by observing expression of flourescent protein, and found to have increased transcription in response to increasing concentrations of potassium nitrate.Notes
-Source
E9-ns2 CArG promoter sequence + BHN + BBaK216005 + BBa_K081014| Access | Instance | Definition |
| public public | BBa_K774001 BBa_K081014 | M-B BBa_K081014 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K774001 BBa_K081014 | 1,208 217,990 | M-B BBa_K081014 |