Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Team Goettingen
Date created: 2012-09-20 11:00:00
Date modified: 2015-05-08 01:13:17
yhjH under the control of constitutive promoter J23100
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K777122_sequence (Version 1) |
Description
The product of this gene is a phosphodiesterase which is involved in regulating the levels of c-di-GMP, a bacterial signaling molecule. High levels of c-di-GMP promote biofilm synthesis and inhibit flagella by binding to YcgR which then acts as a "brake" on FliG and FliM. Levels of c-di-GMP are elevated and motility is reduced in yhjH-mutants.Here we used 3 different constitutive Anderson promoters from the 2006 Berkeley group to test how different levels of constitutive yhjH expression affect the motility of E. coli.
Notes
- yhjH was amplified from genomic DNA of E. coli DH10B via PCR using the following primers:
- Fwd: 5'-gtttcttcgaattcgcggccgcttctagatgataaggcaggttatccagc-3'
- Rev: 5'-gtttcttcctgcagcggccgctactagtattatagcgccagaaccgccgtattcagc-3'
Source
- yhjH was amplified from genomic DNA of E. coli str. K-12 substr. DH10B, complete genome (CP000948.1).
- J23100 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
| Access | Instance | Definition |
| public public public | BBa_J23100 BBa_B0034 BBa_K777121 | BBa_J23100 BBa_B0034 BBa_K777121 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23100 BBa_B0034 BBa_K777121 | 1,35 44,55 62,829 | BBa_J23100 BBa_B0034 BBa_K777121 |