Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Kevin Chen
Date created: 2012-10-02 11:00:00
Date modified: 2015-05-08 01:13:18
[R0010][B0034] - Cohesin II-FliC Insertion Chimera
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K781003_sequence (Version 1) |
Description
This is type II cohesin from Clostridium Thermocellum as a chimeric insertion into flagellin.Notes
The linker and D3 overlap sequences are standard for our parts. By digesting the plasmid with the two inner enzymes, SpeI and NheI for our BB-2 standard parts, you can purify and then PCR overlap extend using the following protocol to result in a brand new chimera.Source
The cohesin portion was PCR amplified from a plasmid provided by Dr. Steven Smith of the Queen's University Department of Biomedical and Molecular Sciences. The amplified part was used for PCR overlap extension into the flagellin variable domain.| Sequence Annotation | Location | Component / Role(s) |
| BBa_R0010 end of LacI coding region (inactive) CAP binding site -35 -10 LacI binding site start conserved BBa_B0034 Flagellin Flagellin Cohesin II Frame shift error | 1,200 1,88 89,126 137,142 161,166 166,200 173,173 213,216 209,220 230,975 1538,2288 998,1517 998,998 | engineered_region feature/BioBrick feature/cds CDS non_covalent_binding_site feature/binding promoter feature/promoter promoter feature/promoter non_covalent_binding_site feature/binding start_codon feature/start feature/conserved conserved_region feature/BioBrick engineered_region feature/protein CDS CDS feature/protein CDS feature/protein sequence_feature feature/misc |