Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Kevin Chen
Date created: 2012-10-02 11:00:00
Date modified: 2015-05-08 01:13:18
[R0010][B0034] - LinB Dehalogenase-FliC Insertion Chimera
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K781005_sequence (Version 1) |
Description
LinB is a dehalogenase enzyme. It degrades haloalkanes which are toxic pollutants that accumulate in food chains. This part has the LinB incorporated at an insertion site.Notes
The linker and D3 overlap sequences are standard for our parts. By digesting the plasmid with the two inner enzymes, SpeI and NheI for our BB-2 standard parts, you can purify and then PCR overlap extend using the following protocol to result in a brand new chimera.Source
This part was amplified from a plasmid provided to us by another research lab. It was then used for PCR overlap extension into the flagellin variable domain using the part K781000 as a template.| Sequence Annotation | Location | Component / Role(s) |
| BBa_R0010 end of LacI coding region (inactive) CAP binding site -35 -10 LacI binding site start conserved BBa_B0034 Flagellin Linker 1 LinB Linker 2 Flagellin | 1,200 1,88 89,126 137,142 161,166 166,200 173,173 213,216 209,220 230,976 977,997 998,1882 1883,1902 1903,2653 | engineered_region feature/BioBrick feature/cds CDS feature/binding non_covalent_binding_site feature/promoter promoter feature/promoter promoter non_covalent_binding_site feature/binding feature/start start_codon feature/conserved conserved_region engineered_region feature/BioBrick CDS feature/protein CDS feature/protein feature/protein CDS CDS feature/protein feature/protein CDS |