Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Marie Burghard, Jascha Diemer, Philipp Rottmann, Rene Sahm, Arne Wehling
Date created: 2012-09-03 11:00:00
Date modified: 2015-05-08 01:13:26
Arabinose regulated RBS-PhoA-His6tag-pNBEst13-Myctag-EstA
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K808032_sequence (Version 1) |
Description
This a a composite of 2 different BioBricks by an standard assembly, forming a scar in between.It contains the following units:
AraC-AraO2-AraO1-AraPromo(PBAD)- RBS-PhoA-His6tag-pNBEst13-Myctag-EstA
which is similiar to BBa_K808000 & BBa_K808030
Its a regulatory system, being induced by adding 1 to 2% of mw% L-Arabinose. It is made for regulating the cell surface Expression of our PET cleaving enzyme pNB-Est13, which is anchored c-terminal to EstA.
Notes
We were not able to add this part as an composite due to missing links and unedited registry pages. Necessary informations are featured in the sequence.Source
assembled GENEART synthesized product(BBa_K808030)and cloned regulatory unit (BBa_K808000), deriving from an expression system (pBAD-vector)| Sequence Annotation | Location | Component / Role(s) |
| AraC ara O2 ara O1 ara promotor scar BBa_B0029 6histag BBa_K808028 BBa_K808026 myctag BBa_K808027 stop stop | 1,879 909,924 1066,1077 1153,1181 1211,1218 1220,1234 1304,1321 1241,1303 1322,2791 2792,2821 2822,4255 4256,4258 4259,4261 | CDS feature/protein feature/binding non_covalent_binding_site feature/binding non_covalent_binding_site feature/promoter promoter sequence_feature feature/misc feature/rbs ribosome_entry_site feature/tag tag CDS feature/protein CDS feature/protein feature/tag tag feature/protein CDS stop_codon feature/stop stop_codon feature/stop |