BBa_K814003

BBa_K814003 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K814003

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Nolan Kennedy, Michelle Goettge, Grayson Wawrzyn

Date created: 2012-10-02 11:00:00

Date modified: 2015-05-08 01:13:28

shinorine non-ribosomal peptide synthase (NRPS) generator

Details

• Types: DnaRegion
• Roles: Generator; engineered_region
• Sequences: BBa_K814003_sequence (Version 1)

Description

Our research has focused on two novel biosynthetic pathways found in two distinct algal species. A pathway ending in the production of two UV-protective compounds, shinorine and mycosporine-glycine, was cloned from Anabaena varibalis. NRPS catalyzes the final step in the pathway, converting mycosporine-glycine into shinorine.

This part includes a modified constitutive lac promoter (lacP???), an RBS and the open reading frame of NRPS. This part can be used to express the shinorine NRPS in E. coli.

Notes

Genomic DNA was obtained from the Brett Barney Lab (University of Minnesota) for A. variabilis. From here, PCR primer extension was used to clone the ORF into a BioBrick???-compatible vector containing lacPmod, an RBS, and a multiple cloning site flanked by the BioBrick??? sites.

Source

Anabaena variabilis ATCC 29413 genomic DNA (accession NC_007413.1)

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
lacP'	1,127	promoter, feature/promoter
RBS	128,133	ribosome_entry_site, feature/rbs
NRPS	140,2806	CDS, feature/protein

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K814003/1

Member of these Collections

• iGEM Parts Registry