Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Nolan Kennedy, Michelle Goettge, Grayson Wawrzyn
Date created: 2012-10-02 11:00:00
Date modified: 2015-05-08 01:13:28
negative control for mycosporine-like amino acid biosynthesis
| Types | DnaRegion |
| Roles | Other sequence_feature |
| Sequences | BBa_K814006_sequence (Version 1) |
Description
Our research has focused on two novel biosynthetic pathways found in two distinct algal species. A pathway ending in the production of two UV-protective compounds, shinorine and mycosporine-glycine, was cloned from Anabaena varibalis. DHQS catalyzes the first step in the pathway, converting sedoheptulose-7-phosphate into dehydroquinate. Dehydroquinate-O-methyltransferase (O-MT) then generates 4-deoxygadusol, which is converted to mycosprine-glycine by ATP-grasp (ATPG). Finally, mycosporine-glycine is converted into shinorine by a non-ribosomal peptide synthase (NRPS).This composite part contains protein generators for DHQS and ATPG. The part is non-functional and was used by the 2012 Minnesota iGEM team as a negative control when testing biosynthesis of mycosporine-like amino acids by the parts BBa_K814004 and BBa_K814005.
Notes
Genomic DNA was obtained from the Brett Barney Lab (University of Minnesota) for A. variabilis. From here, PCR primer extension was used to clone each individual gene (seven in total) with flanking restriction digest sites to be used for vector cloning. Each gene was cloned into an individual pUCBB BioBrick??? cloning vector containing a modified constitutive lac promoter (lacP')and RBS. From here, the resulting generators were stacked into a BioBrick??? vector using classical assembly techniques.Source
All open reading frames contained within this part came from Anabaena variabilis ATCC 29413 genomic DNA (accession NC_007413.1).| Access | Instance | Definition |
| public public | BBa_K814000 BBa_K814001 | BBa_K814000 BBa_K814001 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K814000 BBa_K814001 | 1,1445 1454,3042 | BBa_K814000 BBa_K814001 |