BBa_K824009

BBa_K824009 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K824009

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Steven Allen

Date created: 2012-10-01 11:00:00

Date modified: 2015-07-24 01:19:47

Arsenic Promoter with GFP Reporter (Arsenic Detector)

Details

• Types: DnaRegion
• Roles: engineered_region; Composite
• Sequences: BBa_K824009_sequence (Version 1)

Description

We digested the promoter responsive to Arsenic(BBa_K174015) with EcoRI and SpeI and digested the GFP plasmid (BBa_I13401) with XbaI and PstI. The promoter was Chloramphenicol resistant and the GFP was Ampicillin resistant. These digested fragments were mixed and ligated to the provided, linearized pSB3C1 plasmid. The ligation mix was grown under Chloramphenicol selection. The resulting colonies were tested for responsive GFP production after the addition of Cadmium Chloride since the promoter is responsive to multiple heavy metals.

Notes

none

Source

This is a composite part formed from parts BBA_K174015 and BBa_I13401.

Components

Access	Instance	Definition
public	BBa_J33201	BBa_J33201
public	BBa_I13401	BBa_I13401

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_J33201	1,518	BBa_J33201
BBa_I13401	525,1381	BBa_I13401

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K824009/1

Member of these Collections

• //function/sensor/metal
• iGEM Parts Registry