Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Brian Smith
Date created: 2012-10-01 11:00:00
Date modified: 2016-02-10 11:27:13
Spider Silk Generator - 4x "B" Silk Construct with His Tag
| Types | DnaRegion |
| Roles | Generator engineered_region |
| Sequences | BBa_K844016_sequence (Version 1) |
Description
Protein generator part for Spider Silk. Protein is a 4x fusion of spider silk "B" subunits, which were codon optimized to use a reduced set of tRNA codons (1 to 2 codons per amino acid). Contains a lactose/IPTG inducible , RBS, and a 10x-His tag for purification of protein.IMPORTANT NOTE: This part uses Assembly Standard #23 (Silver Fusion) scar sites, which the sequence data below will not reflect (it shows Assembly Standard #10 scars).
Notes
none.Source
Silver Fusion assembly of basic parts| Access | Instance | Definition |
| public public public public public public | BBa_K208010 BBa_K844008 BBa_K844004 BBa_K844004 BBa_K844004 BBa_K844000 | BBa_K208010 BBa_K844008 BBa_K844004 BBa_K844004 BBa_K844004 BBa_K844000 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K208010 BBa_K844008 BBa_K844004 BBa_K844004 BBa_K844004 BBa_K844000 | 1,220 227,433 442,645 654,857 866,1069 1078,1113 | BBa_K208010 BBa_K844008 BBa_K844004 BBa_K844004 BBa_K844004 BBa_K844000 |