Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Erik Lundin
Date created: 2012-09-22 11:00:00
Date modified: 2015-05-08 01:13:37
Low copy BioBrick standard vector
| Types | DnaRegion |
| Roles | plasmid_vector Plasmid_Backbone |
| Sequences | BBa_K864000_sequence (Version 1) |
Description
pSB4A15 is a BioBrick standard vector with low copy pSC101 replication origin (~5 copies per cell) and ??? antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22.Notes
The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is.The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone.
The pSC101 origin of replication is flaked by NheI and MluI restriction sites for easy switching of origin of replication.
Source
Based on pSB3T5| Sequence Annotation | Location | Component / Role(s) |
| -10 -10 -35 -35 repA ampR BBa_P1002 Mutated A>T to remove SpeI Lambda Red forward primer Lambda Red reverse primer VF2 VR RBS Tandem repeat Tandem repeat Repeat "4" Tandem repeat Repeat "5" pSC101 ori tonB terminator Lux terminator T3 terminator rrnC terminator | 903,908 3387,3392 881,886 3413,3418 939,1889 2509,3369 867,867 2433,2450 18,38 3495,3514 116,135 3374,3378 790,809 767,788 862,880 820,841 891,912 190,2405 47,115 2454,2499 3515,3592 136,175 | feature/promoter promoter feature/promoter promoter feature/promoter promoter feature/promoter promoter CDS feature/cds sequence_feature feature/misc feature/mutation sequence_alteration primer_binding_site feature/primer_binding primer_binding_site feature/primer_binding primer_binding_site feature/primer_binding feature/primer_binding primer_binding_site ribosome_entry_site feature/rbs sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc feature/misc sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc sequence_feature sequence_feature feature/misc |