Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Erik Lundin
Date created: 2012-09-22 11:00:00
Date modified: 2015-05-08 01:13:37
Low copy BioBrick standard vector
| Types | DnaRegion |
| Roles | Plasmid_Backbone plasmid_vector |
| Sequences | BBa_K864001_sequence (Version 1) |
Description
pSB4C15 is a BioBrick standard vector with low copy pSC101 replication origin (~5 copies per cell) and chloramphenicol antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22.Notes
The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is.The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone.
The pSC101 origin of replication is flaked by NheI and MluI restriction sites for easy switching of origin of replication.
Source
Based on pSB3T5| Sequence Annotation | Location | Component / Role(s) |
| Lambda Red reverse primer tonB terminator VR rrnC terminator pSC101 ori Tandem repeat Tandem repeat Tandem repeat Repeat "4" Mutated A>T to remove SpeI -35 Repeat "5" -10 repA Lambda Red forward primer T3 terminator Lux terminator CmR BBa_P1004 Promoter P22 late terminator VF2 | 18,38 47,115 116,135 136,175 190,2405 767,788 790,809 820,841 862,880 867,867 881,886 891,912 903,908 939,1889 2433,2450 3342,3419 2454,2499 2506,3168 3235,3261 3251,3321 3322,3341 | primer_binding_site feature/primer_binding feature/stem_loop stem_loop primer_binding_site feature/primer_binding feature/stem_loop stem_loop feature/misc sequence_feature sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc feature/misc sequence_feature feature/promoter promoter feature/misc sequence_feature feature/promoter promoter CDS feature/cds feature/primer_binding primer_binding_site stem_loop feature/stem_loop sequence_feature feature/misc feature/cds CDS promoter feature/promoter feature/stem_loop stem_loop primer_binding_site feature/primer_binding |